Combined cloud:a mixture of voluntary cloud and reserved instance marketplace

Voluntary cloud is a new paradigm of cloud computing.It provides an alternative selection along with some well-provisioned clouds.However,for the uncertain time span that participants share their computing resources in voluntary cloud,there are some challenging issues,i.e.,fluctuation,under-capacity and low-benefit.In this paper,an architecture is first proposed based on Bittorrent protocol.In this architecture,resources could be reserved or requested from Reserved Instance Marketplace and could be accessed with a lower price in a short circle.Actually,these resources could replenish the inadequate resource pool and relieve the fluctuation and under-capacity issue in voluntary cloud.Then,the fault rate of each node is used to evaluate the uncertainty of its sharing time.By leveraging a linear prediction model,it is enabled by a distribution function which is used for evaluating the computing capacity of the system.Moreover,the cost optimization problem is investigated and a computational method is presented to solve the low-benefit issue in voluntary cloud.At last,the system performance is validated by two sets of simulations.And the experimental results show the effectiveness of our computational method for resource reservation optimization

Data and Interaction Oriented Workflow Execution 1

Abstract. For better supervising workflow performance, the data elements engaged in workflow execution are classified into application data and process data. Taking advantage of HyperSet and Nested HyperSet, the rationale of domain-specified control and interaction is discussed and a fashion directing the hierarchical interaction is explored based on the domain-specified disciplines. The conclusions are presented at last.

A DNA vaccine candidate delivered by an electroacupuncture machine provides protective immunity against SARS-CoV-2 infection

Abstract A major challenge in the use of DNA vaccines is efficient DNA delivery in vivo. Establishing a safe and efficient electric transfer method is the key to developing rapid DNA vaccines against emerging infectious diseases. To overcome the complexity of designing new electric transfer machines for DNA delivery, a clinically approved electric transfer machine could be considered as an alternative. Here, we report an electroacupuncture machine-based method for DNA vaccine delivery after intramuscular injection of the COVID-19 DNA vaccine. The S gene of SARS-CoV-2 in the pVAX1 plasmid (pSARS2-S) was used as an antigen in this study. We optimized the clinically used electroacupuncture machine settings for efficient induction of the neutralizing antibody titer after intramuscular injection of pSARS2-S in mice. We found that pSARS2-S immunization at 40 Vpp for 3–5 s could induce high neutralizing antibody titers and Th1-biased immune responses. IFN-γ/TNF-α-secreting CD4+ and CD8+ T cells were also observed in the DNA vaccination group but not in the recombinant protein vaccination group. T-cell epitope mapping shows that the major reactive epitopes were located in the N-terminal domain (a.a. 261–285) and receptor-binding domain (a.a. 352–363). Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. In the preclinical toxicology study, blood biochemistry, hematology, and DNA persistence analysis reveal that the DNA delivery method is safe. Furthermore, the raised antisera could also cross-neutralize different variants of concern. These findings suggest that DNA vaccination using an electroacupuncture machine is feasible for use in humans in the future

Turn-on Phosphorescent Chemodosimeter for Hg²⁺ Based on a Cyclometalated Ir(III) Complex and Its Application in Time-Resolved Luminescence Assays and Live Cell Imaging

A novel “turn-on” phosphorescent chemodosimeter based on a cyclometalated Ir­(III) complex has been designed and synthesized, which displays high selectivity and sensitivity toward Hg²⁺ in aqueous media with a broad pH range of 4–10. Furthermore, by time-resolved photoluminescence techniques, some interferences from the short-lived background fluorescence can be eliminated effectively and the signal-to-noise ratio of the emission detection can be improved distinctly by using the chemodosimeter. Finally, the chemodosimeter can be used to monitor Hg²⁺ effectively in living cells by confocal luminescence imaging